Test Description

CosMx SMI is the first high-plex in situ analysis platform to provide spatial multiomics with formalin-fixed paraffin-embedded (FFPE) and fresh frozen (FF) tissue samples at cellular and subcellular resolution. CosMx SMI enables rapid quantification and visualization of up to 1,000 RNA and 64 validated protein analytes. The CosMx Spatial Molecular Imager (SMI) allows researchers to comprehensively map single cells in their native environment and extract deeper biological insights from a single experiment. Researchers are able to understand the different cell types, how cell behave with one another and their purpose which is vital in enhancing the ability of interpreting the biology and disease.


How does it works?

CosMx SMI is an integrated system with mature cyclic fluorescent in situ hybridization (FISH) chemistry, high-resolution imaging readout, interactive data analysis and visualization software. Streamlined and simple workflow that integrates with standard ISH protocol with no need for tissue expansion or clearing, cDNA synthesis or amplification. Go from sample to result faster.




CosMx Spatial Molecular Imager is the most flexible and robust spatial single-cell imaging platform for:

  • Cell Atlas and Characterization: Define cell types, cell states, tissue microenvironment phenotypes, and gene expression networks within spatial context.
  • Cell-cell Interaction: Understand biological process controlled by ligand-receptor interactions.
  • Spatial Biomarkers: Quantify change in gene expression based on treatment and identify single-cell subcellular biomarkers with spatial context.


Ordering Information

Turnaround time: Varies (~ 6-12 weeks); contact us for more details


Sample Requirement:


  • Unstained FFPE sections mounted on charged slide (Matsunami PLATINUM PRO slides preferred. Fisherbrand™ Superfrost™ Plus/Leica BOND Plus slides can be used);
  • Fresh frozen sections do not give optimal results, but can be performed;
  • Thickness of section at 5 μm;
  • Dimension of the mounted section as shown in green. Section outside of green shaded area is not scanned.


Slide Dimension:




References & Materials